The growing prevalence of antibiotic-resistant bacterial infections, notably methicillin-resistant Staphylococcus aureus (MRSA), presents a big public well being concern. Well timed detection of MRSA is essential to allow immediate medical intervention, restrict its unfold, and scale back antimicrobial resistance. Right here, we introduce a miniaturized nano-sieve gadget that includes a pneumatically-regulated chamber for extremely environment friendly MRSA purification from human plasma samples. By utilizing packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we achieved an on-chip focus issue of ∼15-fold for MRSA. We built-in this gadget with recombinase polymerase amplification (RPA) and clustered repeatedly interspaced brief palindromic repeats (CRISPR)-Cas detection system, leading to an on-chip restrict of detection (LOD) of roughly 100 CFU mL⁻¹. This developed strategy gives a fast, exact, and centrifuge-free answer appropriate for point-of-care diagnostics, with the potential to considerably enhance affected person outcomes in resource-limited medical situations.