The multi-dimensional potential vitality floor (PES) of a nanoparticle, similar to a naked cluster of steel atoms, controls each the construction and dynamic behaviour of the particle. These properties are the topic of quite a few theoretical simulations. Nonetheless, quantitative experimental measurements of crucial PES parameters are wanted to manage the fashions employed within the theoretical work. Experimental measurements of parameters are at the moment few in quantity, whereas mannequin parameters taken from bulk programs might not be appropriate for nanosystems. Right here we describe a brand new measurement methodology, wherein the isomer constructions of a single deposited nanocluster are obtained frame-by-frame in an aberration-corrected Scanning Transmission Electron Microscope (ac-STEM) in Excessive Angle Annular Darkish Discipline (HAADF) mode. A number of gold clusters containing 309 ± 15 atoms had been analysed individually after deposition from a mass-selected cluster supply onto an amorphous carbon movie. The principle isomers recognized are icosahedral, decahedral and face-centred-cubic (the majority construction), alongside many amorphous (glassy) constructions. The outcomes, that are broadly according to static ac-STEM measurements of an ensemble of such clusters, open the way in which to dynamic measurements of many alternative nanoparticles of various sizes, shapes and compositions.