The cooperative relationship between biomolecules and nanomaterials makes up an attractive story about nanoscale chiral sensing and separation. Biomolecules are thought of a superb chirality ‘donor’ to develop chiral sensors and separation programs. Nature has endowed biomolecules with mysterious chirality. Varied nanomaterials with particular physicochemical attributes can understand the transmission and amplification of this chirality. We concentrate on highlighting the benefits of combining biomolecules and nanomaterials in nanoscale chirality. To boost the sensors’ detection sensitivity, novel cooperation approaches between nanomaterials and biomolecules have attracted large consideration. Furthermore, progressive biomolecule-based nanocomposites possess nice significance in creating chiral separation programs with improved assay efficiency. This assessment describes the formation of a community based mostly on nanomaterials and biomolecules primarily together with DNA, proteins, peptides, amino acids, and polysaccharides. We hope this story will document the perpetual relation between biomolecules and nanomaterials in nanoscale chirality.